Publications

ORCID ID: 0000-0003-3384-5328

ResearcherID: AAW-4815-2021

Research Papers

• Sedláček, I., Holochová, P., Sedlář, K. , Staňkové E., Šedo O., Kralova S., Umair M., Koublova V., Švec P. Two new psychrotolerant Massilia species inhibit plant pathogens Clavibacter and Curtobacterium. Sci Rep 15, 26134 (2025). https://doi.org/10.1038/s41598-025-09734-0
• Medeiros W., Kralova S., Oliveira V, Ziemert N., Sehnal L. Antarctic bacterial natural products: from genomic insights to drug discovery. Nat. Prod. Rep., 2025,42, 774-787
• Vaibarova, V., Kralova, S., Palikova, M. et al. Genetic and phenotypic diversity of Flavobacterium psychrophilum isolates from Czech salmonid fish farms. BMC Microbiol. 2024. 24: 352. 

• Sedláček I, Holochová P, Busse H-J, Koublová V, Králová S, Švec P, Sobotka R, Staňková E, Pilný J, Šedo O, Smolíková J, Sedlář K. Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. Microorganisms 2022, 10: 704.

• Švec P, Králová S*, Staňková E, Holochová P, Sedlář K, Koudelková S, Krsek D, Grzesiak J, Šedo O, Váczi P, Urvashi, Gupta V, Sood U, Lal R, Korpole S, Sedláček I. Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica. Int. J Syst. Evol. Microbiol. 2022. 72(4): 005309.

• Králová S*, Davidova-Gerzova L, Valcek A, Bezdicek M, Rychlik I, Rezacova V, Cizek A. Paraphocaeicola brunensis gen. nov., sp. nov., carrying two variants of nimB resistance gene from Bacteroides fragilis, and Caecibacteroides pullorum gen. nov., sp. nov., two novel genera isolated from chicken caeca. Microbiol. Spectr. 2022. 10(1):e0195421.

• Králová S# , LaFrentz BR#, Burbick CR, Alexander TL, Phillips CW, Griffin MJ, Waldbieser GC, García JC, de Alexandre Sebastião F, Soto E, Loch TP, Liles MR, Snekvik KR. The fish pathogen Flavobacterium columnare represents four distinct species: Flavobacterium columnare, Flavobacterium covae sp. nov., Flavobacterium davisii sp. nov. and Flavobacterium oreochromis sp. nov., and emended description of Flavobacterium columnare. Syst. Appl. Microbiol. 2021. 45(2):126293. # Authors contributed equally to this work

• Králová S*, Davidova-Gerzova L, Valcek A, Bezdicek M, Rychlik I, Rezacova V, Cizek A. Paraphocaeicola brunensis gen. nov., sp. nov., carrying two variants of nimB resistance gene from Bacteroides fragilis, and Caecibacteroides pullorum gen. nov., sp. nov., two novel genera isolated from chicken caeca. Microbiol. Spectr. 2022. 10(1):e0195421.

• Kovařovic V, Sedláček I, Petráš P, Králová S, Mašlaňová I, Švec P, Neumann-Schaal M, Botka T, Gelbíčová T, Staňková E, Doškař J, Pantůček R. (2022) Staphylococcus ratti sp. nov. Isolated from a Lab Rat. Pathogens 11(1): 51.

• Roldán D.M, Costa A, Králová S, Busse H.-J, Amarelle V, Fabiano E, Menes R.J. Paenibacillus farraposensis sp. nov., isolated from a root nodule of Arachis villosa. Int. J Syst. Evol. Microbiol. 2022. 72(3): 005294.

• Králová S*, Busse H-J, Bezdíček M, Sandoval-Powers M, Nykrýnová M, Staňková E, Krsek D, Sedláček I. Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov., Two Multidrug-Resistant Psychrotrophic Species Isolated From Antarctica. Front. Microbiol. 2021. 12:729977.

• Bezdicek M, Nykrynova M, Sedlar K, Kralova S, Hansliková J, Komprdova A, Skutkova H, Kocmanova I, Mayer J, Lengerova M. Rapid high-resolution melting genotyping scheme for Escherichia coli based on MLST derived single nucleotide polymorphisms. Sci. Rep. 2021. 11:16572.

• Sandoval-Powers M, Králová S, Nguyen G-S, Fawwal DV, Degnes K, Lewin AS, Klinkenberg G, Wentzel A, Liles MR. Streptomyces poriferorum sp. nov., a novel marine sponge-derived Actinobacteria species expressing anti-MRSA activity. Syst. Appl. Microbiol. 2021. 44(5):126244.

• Králová S, Sandoval-Powers M, Fawwal DV, Degnes KF, Lewin AS, Klinkenberg G, Nguyen G-S, Liles M, Wentzel A. Streptomyces tardus sp. nov., a slow-growing actinobacterium producing candicidin, isolated from sediments of the Trondheim fjord. Front. Microbiol. 2021. 12:2131.

• Sedláček I, Holochová P, Sobotka R, Busse H-J, Švec P, Králová S, Šedo O, Pilný J, Staňková E, Koublová V, Sedlář K. Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov. Microbiol. Spectr. 2021. 9(1): e00452-21.

• Roldán DM, Kyrpides N, Woyke T, Shapiro N, Whitman WB, Králová S, Sedláček I, Busse H-J, Menes RJ. Hymenobacter caeli sp. nov., an airborne bacterium isolated from King George Island, Antarctica. Int. J Syst. Evol. Microbiol. 2021. 71(6):004838.

• Švec P, Kosina M, Zeman M, Holochová P, Králová S, Němcová E, et al. Pseudomonas karstica sp. nov. and Pseudomonas spelaei sp. nov., isolated from calcite moonmilk deposits from caves. Int. J Syst. Evol. Microbiol. 2020;70(9):5131–40.

• Rapoport D, Sagova-Mareckova M, Sedláček I, Provaznik J, Králová S, Pavlinic D, Benes V, Kopecky J. Trebonia kvetii gen. nov., sp. nov., an acidophilic actinobacterium, and proposal of the new actinobacterial family Treboniaceae fam. nov. Int. J Syst. Evol. Microbiol. 2020. 70(9): 5106–14.

• Roldán DM, Kyrpides N, Woyke T, Shapiro N, Whitman WB, Králová S, Němcová E, Micenková L, Urvashi, Gupta V, Sood U, Lal R, Korpole S, Sedláček I. Hymenobacter artigasi sp. nov., isolated from air sampling in maritime Antarctica. Int. J Syst. Evol. Microbiol. 2020. 70(9): 4935–41.

• Holochová P, Mašlaňová I, Sedláček I, Švec P, Králová S, Kovařovic V, Busse H-J, Staňková E, Barták M, Pantůček R. Description of Massilia rubra sp. nov., Massilia aquatica sp. nov., Massilia mucilaginosa sp. nov., Massilia frigida sp. nov., and one Massilia genomospecies isolated from Antarctic streams, lakes and regoliths. Syst. Appl. Microbiol. 2020. 43(5):126112.

• Sedláček I, Pantůček R, Zeman M, Holochová P, Šedo O, Staňková E, Švec P, Králová S, Vídeňská P, Micenková L, Urvashi, Korpole S, Lal R. Hymenobacter terrestris sp. nov. and Hymenobacter lapidiphilus sp. nov., isolated from regoliths in Antarctica. Int. J Syst. Evol. Microbiol. 2020;70(12):6364–72.

• Nováková D, Švec P, Zeman M, Busse H-J, Mašľaňová I, Pantuček R, Králová S, Krištofová L, Sedláček I. Pseudomonas leptonychotis sp. nov. isolated from Weddell seals in Antarctica. Int. J. Syst. Evol. Microbiol. 2020. 70(1):302–308.

• Sedláček I, Pantůček R, Holochová P, Králová S, Staňková E, Vrbovská V, Šedo O, Švec P, Busse H.-J. Hymenobacter humicola sp. nov., isolated from soils in Antarctica. Int. J Syst. Evol. Microbiol. 2019. 69(9): 2755–61.

• Králová S*, Busse H-J, Švec P, Mašlaňová I, Staňková E, Barták M, Sedláček I. Flavobacterium circumlabens sp. nov. and Flavobacterium cupreum sp. nov., two psychrotrophic species isolated from Antarctic environmental samples. Syst. Appl. Microbiol. 2019. 42(3):291-301.

• Sedláček I, Pantuček R, Králová S, Mašlaňová I, Holochová P, Staňková E, Vrbovská V, Švec P, Busse H-J. Hymenobacter amundsenii sp. nov. resistant to ultraviolet radiation, isolated from regoliths in Antarctica. Syst. Appl. Microbiol. 2019. 42(3):284-290.

• Králová S*, Švec P, Busse H-J, Staňková E, Váczi P, Sedláček I. Flavobacterium chryseum sp. nov. and Flavobacterium psychroterrae sp. nov., novel environmental bacteria isolated from Antarctica. Int. J Syst. Evol. Microbiol. 2018. 68(10): 3132-3139.

• Mašlaňová I, Wertheimer Z, Sedláček I, Švec P, Indráková A, Kovařovic V, Schumann P, Spröer C, Králová S, Šedo O, Krištofová L, Vrbovská V, Füzik T, Petráš P, Zdráhal Z, Ružičková V, Doškař J, Pantuček R. Description and Comparative Genomics of Macrococcus caseolyticus subsp. hominis subsp. nov., Macrococcus goetzii sp. nov., Macrococcus epidermidis sp. nov., and Macrococcus bohemicus sp. nov., Novel Macrococci From Human Clinical Material With Virulence Potential and Suspected Uptake of Foreign DNA by Natural Transformation. Front. Microbiol. 2018. Article 1178.

• Pantůček R, Sedláček I, Indráková A, Vrbovská V, Mašľanová I, Kovařovič V, Švec P, Králová S, Krištofová L, Kekláková J, Petráš P, Doškař J. Staphylococcus edaphicus sp nov., isolated in Antarctica, harbors the mecC gene and genomic islands with a suspected role in adaptation to extreme environments. Appl. Environ. Microbiol. 2018. 84(2): e01746–17.

• Králová S*. Role of fatty acids of Antarctic psychrophilic Flavobacterium spp. Syst. Appl. Microbiol. 2017. 40(6):329-333.

• Sedláček I, Pantůček R, Králová S, Mašlaňová I, Holochová P, Staňková E, Sobotka R, Barták M, Busse H-J, Švec P. Mucilaginibacter terrae sp. nov., isolated from Antarctic soil. Int. J. Syst. Evol. Microbiol. 2017. 67(10):4002-4007.

• Švec P, Králová S, Busse H-J, Kleinhagauer T, Kýrová K, Pantůček R, Mašlaňová I, Staňková E, Němec M, Holochová P, Barták M, Sedláček I. Pedobacter psychrophilus sp. nov., isolated from Antarctic fragmentary rock. Int. J. Syst. Evol. Microbiol. 2017. 67(8):2538-2543.

• Sedláček I, Králová S, Kýrová K, Mašlaňová I, Busse H-J, Staňková E, Vrbovská V, Němec M, Barták M, Holochová P, Švec P, Pantůček R. Red-pink pigmented Hymenobacter coccineus sp. nov., Hymenobacter lapidaries sp. nov., and Hymenobacter glacialis sp. nov. isolated from rocks in Antarctica. Int. J. Syst. Evol. Microbiol. 2017. 67(6):1975-1983.

• Švec P, Králová S, Busse H-J, Kleinhagauer T, Pantůček R, Mašlaňová I, Cnockaert M, Vandamme P, Staňková E, Gelbíčová T, Holochová P, Barták M, Kýrová K, Sedláček I. Pedobacter jamesrossensis sp. nov., Pedobacter lithocola sp. nov., Pedobacter mendelii sp. nov., and Pedobacter petrophilus sp. nov., isolated from Antarctic environment Int. J. Syst. Evol. Microbiol. 2017. 67(5):1499-1507.

• Kýrová K, Sedláček I, Pantůček R, Králová S, Holochová P, Mašlaňová I, Staňková E, Kleinhagauer T, Gelbíčová T, Sobotka R, Švec P, Busse H-J Rufibacter ruber sp. nov., isolated from fragmentary rock. Int. J. Syst. Evol. Microbiol. 2016. 66(11):4401 4405.

• Králová S*, Staňková E, Sedláček I. Classification of Aeromonas spp. isolated from water and clinical sources and distribution of virulence genes. Folia Microbiol. 2016. 61(6): 513-521.

• Švec P, Černohlávková J, Busse H-J, Vojtková H, Pantůček R, Cnockaert M, Mašlaňová I, Kralová S, Vandamme P, Sedláček I. Classification of strain CCM 4446T as Rhodococcus degradans sp. nov. Int. J. Syst. Evol. Microbiol. 2015. 65(12):4381-7.

• Pekarová M, Kubala L, Martišková H, Papežiková I, Králová S, Baldus S, Klinke A, Kuchta R, Kadlec J, Kuchtova Z, Kolářová H, Lojek A. The unique role of dietary L-arginine in the acceleration of peritoneal macrophage sensitivity to bacterial endotoxin. Immun. Res. 2013. 56(1):73-84.

Book Chapters

• Skwor T.#, Kralova S.# Aeromonas. In Doyle MP, Diez-Gonzales F and Colin H (eds), Food Microbiology: Fundamentals and Frontiers, 5th Edtition. ASM Press, Washington, DC. 2019. pp 415-435. # Authors contributed equally to this work

Conference Contributions

• Vitkova H. Nykrynova M., Kralova S., Consensus-based Detection Of Biosynthetic Gene Clusters With Application To Ripps From Antarctic Bacteria [poster]. National Bioinformatic Conference (ENBIK) 2025, 9-11.6, 2025, Mala Moravka, Czech Republic.
• Spacek P.,Nykrynova M., Bezdicek M., Skutkova H., Kralova S. Exploring Ribosomal Peptides in Antarctic Extremophiles [talk] . In-depth symposium at the ASM Microbe 2025, 19-23.6. 2025, in Los Angeles, USA. 

• Kralova S., Flieder M., Chen S., Spacek P., Stanic N., Bezdicek M., Zehl M., Zotchev S., Rattei T., Loy A. Exploring the Icy Treasures: Biosynthetic potential of Antarctic bacteria from James Ross Island. In Holuša J., Kadlec M., and Stringer Ch.D. (Eds). Proceedings: Students in Polar and Alpine Research Conference.  p.4. 2023.

• Kralova S., Flieder M., Chen S., Spacek P., Stanic N., Bezdicek M., Zehl M., Zotchev S., Rattei T., Loy A. Exploring the Icy Treasures: Biosynthetic potential of Antarctic bacteria from James Ross Island. [Invited Key Note Lecture]. Students in Polar and Alpine Research Conference (SPARC 2023) held 14.-15. 09. 2023 in Brno, Czech Republic.

• Kralova S., Flieder M., Chen S., Spacek P., Bezdicek M., Dufkova K., Zehl M., Zotchev S., Rattei T., Loy A. Untapped biosynthetic potential of Antarctic soil bacteria [talk]. The 10th FEMS Congress of European Microbiologists (FEMS 2023) held 09.-13. 07. 2023 in Hamburg, Germany.

• Králová S, Chen S, Flieder M, Bezdíček M, Hansliková J, Rattei T, Loy A. Uncovering biosynthetic potential of soil bacteria in Antarctica. [poster] ISME18 held from 14th to 19th August 2022, Lausanne, Switzerland.

• Králová S, Chen S, Flieder M, Špaček P, Bezdíček M, Hansliková J, Zehl M, Zotchev S, Rattei T, Loy A, Antarctic soil bacteria – unprecedented potential to produce novel secondary metabolites. [talk]. 29th Congress of the CSSM held from 15th to 17th September 2022, Brno, Czech Republic.

• Králová S, Busse H-J, Bezdíček M, Nykrýnová M, Staňková E, Kresk D, Sedláček I. Flavobacterium flabelliformis sp. nov. and Flavobacterium geliluteum sp. nov., Two Novel Multidrug Resistant Psychrophilic Species Isolated from Antarctica. [poster]. World Microbe Forum 2021. Held from 20th to 24th June 2021 [online].

• Králová S, Davidová-Geržová L, Medvecký M, Rychlík I, Cigánek M, Čížek A. Two novel fastidious anaerobes from the genus Bacteroides isolated from chicken gastrointestinal tracts. [poster]. In 30th ESCMID. Held from 18th to 21st April 2020 in Paris, France [online].

• Sandoval-Powers M, Pereira A, Zhang Y, Stankey R, Hu Ch, Králová S, Degnes K, Wentzel A, Petrov A, Calderón A, Mead D, Liles M. Culture-Independent and – Dependent Identification and Analysis of Antimicrobial Metabolite-Producing Streptomyces Bacteria. [poster]. The Functional Metagenomic Conference (FMG 2019) held from 16th to 19th June 2019 in Torndheim, Norway.

• Fawwal D, Sandoval-Powers M, Králová S, Degnes KF, Nguyen G-S, Wentzel A, Liles MR. Novel Streptomyces from marine sponges produce metabolites that kill methicillin-resistant Staphylococcus aureus (MRSA). [poster]. AU Student Research Symposium held in March 2019, Auburn, Alabama, USA.

• Králová S, Busse H-J, Švec P, Staňková E, Sedláček I. Two Novel Gliding Flavobacterium Species Isolated from Antarctica. [poster]. ASM Microbe 2018 held from 8th to 10th June 2018 in Atlanta, Georgia, USA.

• Švec P, Králová S, Laichmanová M, Sedláček I. Comparison of typing methods for classification of Antarctic Flavobacterium spp. [poster]. 7th Congress of European Microbiologists. ECCO 2017 held from 13th to 15th September in Brno, Czech Republic.

• Králová S, Staňková E, Sedláček I. Classification of psychrophilic gliding bacteria isolated from Antarctica. [poster]. FEMS 2017 held from 9th to 13th July 2017 in Valencia, Spain.

• Králová S, Staňková E, Sedláček I. Applicability of fatty acid composition for separating clinical and environmental Aeromonas spp. [poster]. ISAP 2017 held from 21st to 23nd June 2017 in Mexico City, Mexico.

• Králová S, Sedláček I. Cold adaptation of cell membrane in Antarctic bacteria. [talk]. Students in Polar and Alpine Research Conference held from 20th to 22nd April 2017 in Brno, Czech Republic.

• Sedláček I, Králová S, Sedláčková M, Kroupová E, Gelbíčová T, Švec P. Classification of Aeromonas popoffii strains isolated from fresh water sources. [poster]. International Congress of Bacteriology and Applied Microbiology, IUMS 2014 held from 27th July to 1st August 2014 in Montréal, Canada.

• Králová S, Gelbicova T, Švec P, Kroupová E, Sedláček I. Classification of Aeromonas spp. isolated from water and clinical sources. [poster]. 11th Symposium of Aeromonas and Plesiomonas, ISAP 2014 held from 25th to 27th June 2014 in Monpellier, France.

• Králová S, Švec P, Kroupová E, Sedláček I. Identification of aeromonads. [talk]. News in Microbiology – Conference for young microbiologists held from 13th to 16th March 2014 in Štrbské Pleso, Slovak Republic.

• Králová S. Identification and typing of aeromonads. [talk]. 26th Congress of the Czechoslovak Society for Microbiology held from 24th to 26th June 2013 in Brno, Czech Republic.